UV-VIS Spectroscopy
UV-VIS Spectroscopy is one of the common tools in many chemist’s tool box. This versatile measurement technique has a secure place in the chemistry lab.
A UV-Vis Spectrometer, sometimes called a UV-Vis Spectrometer, is a device that measures the amount of a substance based on its electromagnetic absorption. Most of the material to be analyzed in the spectrum is liquid. Light irradiation in the visible spectrophotometer is done using tungsten lamp and in UV device with deuterium lamp. The spectrophotometer is used to measure the quality and quantity of a wide range of materials in various scientific and research fields such as chemistry, biochemistry, pharmacy, materials and the environment
Basis of operation of UV-VIS Spectroscopy
UV-Vis Spectroscopy (or Spectrophotometry) is a quantitative technique used to measure how much a chemical substance absorbs light. This is done by measuring the intensity of light that passes through a sample with respect to the intensity of light through a reference sample or blank. This technique can be used for multiple sample types including liquids, solids, thin-films and glass.
Spectrophotometry is a quantitative measurement of the absorption/transmission or reflection of a material as a function of wavelength. Despite being termed UV-Vis, the wavelength range that is typically used ranges from 190 nm up to 1,100 nm in the near-infrared.
Using a spectrophotometer and carrying out absorption/transmission measurements we can determine the amount (or concentration) of a known chemical substance simply, by studying the number of photons (light intensity) that reach the detector. The more a material absorbs light at a specific wavelength, the higher the concentration of the known substance.
Applications of UV-VIS Spectroscopy
UV/Vis spectroscopy is used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules.
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